Abstract: An experimental trial was conducted to ascertain the efficacy of an innovative treatment regimen that comprised a hydraulic cyclone, gravitational oil separator, acidification reservoir, primary and secondary bio-oxidation chambers, and a sedimentation lagoon, all designed to purify saline water emanating from oil fields rich in salt content. The hydrocyclones and separators based on gravity successfully eradicated considerable quantities of total petroleum hydrocarbons, while concurrently enhancing the water's biodegradability, thus establishing the foundation for the elimination of pollutants in subsequent hydrolysis acidification and biological contact oxidation procedures（HA/BCO）. The total hydraulic retention time（HRT） was calibrated to 12 hours. The treatment protocol achieved an average reduction rate of 63.8% for chemical oxygen demand（COD）, 45.3% for ammonia nitrogen（NH₃-N）, 77.7% for total suspended solids（TSS）, and an impressive 97.06% for total petroleum hydrocarbons（TPH）. The integration of halotolerant and lipophilic engineered bacteria into the system further augmented the treatment efficacy.