Abstract: The nitrogen demand and manure nitrogen supply were calculated based on the production amount of fruit,vegetable and tea of different cities and the results of the second national pollution sources survey of Anhui. The manure nitrogen demand for fruit, vegetable and tea planting were evaluated with the manure fertilizer replacement ratios of 0.4, 0.5 and 0.6, respectively. The results showed that the provincial average nitrogen demand for fruit, vegetable and tea planting was 416.78 kg/hm². When the manure fertilizer replacement ratios were 0.4, 0.5 and 0.6, the demand for manure nitrogen were 166.71, 208.39, 250.07 kg/hm². respectively. The provincial average mature nitrogen supply of fruit, vegetable and tea planting was 108.52 kg/hm².. When the manure fertilizer replacement ratios were 0.4, 0.5 and 0.6, the average nitrogen carrying pressure coefficient were 0.651, 0.521 and 0.434 respectively. The study showed that the maximum manure replacement ratios of 16 cities were between 0.121 and 0.657. All the manure provided by livestock and poultry breeding can be used as the fertilizer of fruit, vegetable and tea production in all cities.