Abstract: It is essential to recognize and understand the changing characteristics of ecosystem service value of land use since land has always been vital resource.The recent 25-year’s land use data in the year of 1995,2000,2005,2010,2015,and 2020 of Xi’an was analyzed to study the changing characteristics of ecosystem service value using ArcGIS 10.8 and Excel 365.The results showed that the areas of forest land and construction land were increasing.The areas of farmland,unused land were distinctively decreasing.However,the area of water land was decreasing first,then increasing.The farmland and grassland decreased most by 991.8 km²and 139.59 km²,respectively.The areas of farmland and forest land and grassland and water land and unused land and construction land have changed-307.35 km²,68.48 km²,-72.49 km²,4.73 km²,-0.09 km² and 306.74 km²,respectively in recent 25 years.The ecosystem service value of Xi’an in recent 25 years were about 228.11×10⁸ Yuan,among wich,forest land provided the most value,followed by farmland and water land and grassland and unused land and construction land.The Moran’s I value of the ecosystem service value was more than 0,and its p value was less than 0.001,and Z value was more than 122,which indicated spatially active relationship and aggregation.The study was aimed to provide reference for scientifically planning land use for Xi’an city.